mouse E2F1 shRNA silencing Adenovirus

Name 
Ad-m-E2F1-shRNA
Cat No 
shADV-257649
Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse E2f1.

shADV-257649
Ad-m-E2F1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse E2f1 (E2F transcription factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
E2F-1; mKIAA4009
Description (Vector)
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Gene ID
13555
Gene Name (Vector)
E2F transcription factor 1
Gene Symbol
E2f1
ORF Size (aa)
1293
ORF Size (bp)
1293 bp
RefSeq ID
NM_007891
RefSeq Synonyms
NM_007891, NM_001291105, BC052160,
Species
mouse
UniGene ID
Mm.18036

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.