mouse DGAT1 shRNA silencing Adenovirus

Name
Ad-m-DGAT1-shRNA

Cat No
shADV-257108

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Dgat1.

shADV-257108

Ad-m-DGAT1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Dgat1 (diacylglycerol O-acyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

ARAT; C75990; D15Ertd23e; Dgat

Description (Vector)

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

Gene ID

13350

Gene Name (Vector)

diacylglycerol O-acyltransferase 1

Gene Symbol

Dgat1

ORF Size (aa)

1497

ORF Size (bp)

1497 bp

RefSeq ID

NM_010046

RefSeq Synonyms

NM_010046, BC003717,

Species

mouse

UniGene ID

Mm.22633

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.