mouse DEAF1 shRNA silencing Adenovirus

Name
Ad-m-DEAF1-shRNA

Cat No
shADV-256970

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Deaf1.

shADV-256970

Ad-m-DEAF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Deaf1 (deformed epidermal autoregulatory factor 1 (Drosophila)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

AU042387; C230009B13Rik; NUDR

Description (Vector)

Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm.

Gene ID

54006

Gene Name (Vector)

deformed epidermal autoregulatory factor 1 (Drosophila)

Gene Symbol

Deaf1

ORF Size (aa)

1353

ORF Size (bp)

1353 bp

RefSeq ID

NM_016874

RefSeq Synonyms

NM_016874, NM_001282076, NM_001282073, NM_001282072, BC046399,

Species

mouse

UniGene ID

Mm.28392

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.