mouse DDX42 shRNA silencing Adenovirus

Name 
Ad-m-DDX42-shRNA
Cat No 
shADV-256949
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Ddx42.

shADV-256949
Ad-m-DDX42-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ddx42 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 42). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1810047H21Rik; AW319508; AW556242; B430002H05Rik; RHELP; RNAHP; SF3b125
Description (Vector)
ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.
Gene ID
72047
Gene Name (Vector)
DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Gene Symbol
Ddx42
ORF Size (aa)
2433
ORF Size (bp)
2433 bp
RefSeq ID
NM_028074
RefSeq Synonyms
NM_028074, BC043036,
Species
mouse
UniGene ID
Mm.41367

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.