mouse CHST1 shRNA silencing Adenovirus

Name 
Ad-m-CHST1-shRNA
Cat No 
shADV-255470
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Chst1.

shADV-255470
Ad-m-CHST1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Chst1 (carbohydrate (keratan sulfate Gal-6) sulfotransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2610008E20Rik; AW125896; C6ST; GST-1; Gst1; KSGAL6ST
Description (Vector)
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
Gene ID
76969
Gene Name (Vector)
carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
Gene Symbol
Chst1
ORF Size (aa)
1236
ORF Size (bp)
1236 bp
RefSeq ID
NM_023850
RefSeq Synonyms
NM_023850, BC030667,
Species
mouse
UniGene ID
Mm.38021

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.