mouse CHCHD4 shRNA silencing Adenovirus

Name 
Ad-m-CHCHD4-shRNA
Cat No 
shADV-255367
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Chchd4.

shADV-255367
Ad-m-CHCHD4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Chchd4 (coiled-coil-helix-coiled-coil-helix domain containing 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2410012P20Rik; 2810014D17Rik; AI838740
Description (Vector)
Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (Probable).
Gene ID
72170
Gene Name (Vector)
coiled-coil-helix-coiled-coil-helix domain containing 4
Gene Symbol
Chchd4
ORF Size (aa)
420
RefSeq ID
NM_133928
RefSeq Synonyms
NM_133928, BC019405,
Species
mouse
UniGene ID
Mm.41622

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.