mouse CCND2 shRNA silencing Adenovirus

Name
Ad-m-CCND2-shRNA

Cat No
shADV-254861

Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse Ccnd2.

shADV-254861

Ad-m-CCND2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ccnd2 (cyclin D2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

2600016F06Rik; AI256817; BF642806; C86853; cD2; Vin-1; Vin1

Description (Vector)

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Gene ID

12444

Gene Name (Vector)

cyclin D2

Gene Symbol

Ccnd2

ORF Size (aa)

870

RefSeq ID

NM_009829

RefSeq Synonyms

NM_009829, BC049086,

Species

mouse

UniGene ID

Mm.333406

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.