mouse CASP8 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Casp8.
shADV-254562
Ad-m-CASP8-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Casp8 (caspase 8). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CASP-8; FLICE; MACH; Mch5
Description (Vector)
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Gene ID
12370
Gene Name (Vector)
caspase 8
Gene Symbol
Casp8
ORF Size (aa)
1443
ORF Size (bp)
1443 bp
RefSeq ID
NM_009812
RefSeq Synonyms
NM_009812, NM_001277926, NM_001080126, BC049955, BC006737,
Species
mouse
UniGene ID
Mm.336851
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.