mouse BBS4 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Bbs4.
shADV-253660
Ad-m-BBS4-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Bbs4 (Bardet-Biedl syndrome 4 (human)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AW537059; AW742241; D9Ertd464e
Description (Vector)
BBS4 may be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.
Gene ID
102774
Gene Name (Vector)
Bardet-Biedl syndrome 4 (human)
Gene Symbol
Bbs4
ORF Size (aa)
1563
ORF Size (bp)
1563 bp
RefSeq ID
NM_175325
RefSeq Synonyms
NM_175325, BC145773, BC145771,
Species
mouse
UniGene ID
Mm.200714
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.