mouse B3GAT1 shRNA silencing Adenovirus

Name 
Ad-m-B3GAT1-shRNA
Cat No 
shADV-253523
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse B3gat1.

shADV-253523
Ad-m-B3GAT1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse B3gat1 (beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
0710007K08Rik; AI846286; GlcAT-P; Glcatp; Hnk1
Description (Vector)
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).
Gene ID
76898
Gene Name (Vector)
beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Gene Symbol
B3gat1
ORF Size (aa)
834
ORF Size (bp)
834 bp
RefSeq ID
NM_029792
RefSeq Synonyms
NM_029792, NM_001310766, BC034655, BC023052,
Species
mouse
UniGene ID
Mm.218788

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.