mouse B3GAT1 shRNA silencing AAV

Name
AAV-m-B3GAT1-shRNA

Cat No
shAAV-253523

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse B3gat1.

shAAV-253523

AAV-m-B3GAT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse B3gat1 (beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

0710007K08Rik; AI846286; GlcAT-P; Glcatp; Hnk1

Description (Vector)

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).

Gene ID

76898

Gene Name (Vector)

beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)

Gene Symbol

B3gat1

ORF Size (aa)

834

ORF Size (bp)

834 bp

RefSeq ID

NM_029792

RefSeq Synonyms

NM_029792, NM_001310766, BC034655, BC023052,

Species

mouse

UniGene ID

Mm.218788

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.