mouse ARHGEF4 shRNA silencing Adenovirus

Name 
Ad-m-ARHGEF4-shRNA
Cat No 
shADV-252978
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Arhgef4.

shADV-252978
Ad-m-ARHGEF4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Arhgef4 (Rho guanine nucleotide exchange factor (GEF) 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
9330140K16Rik; Asef; ENSMUSG00000070955
Description (Vector)
Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Gene ID
226970
Gene Name (Vector)
Rho guanine nucleotide exchange factor (GEF) 4
Gene Symbol
Arhgef4
ORF Size (aa)
1455
ORF Size (bp)
1455 bp
RefSeq ID
NM_183019
RefSeq Synonyms
NM_183019, BC139397, BC139371,
Species
mouse
UniGene ID
Mm.41604

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.