mouse ARHGAP17 shRNA silencing Adenovirus

Name
Ad-m-ARHGAP17-shRNA

Cat No
shADV-252934

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Arhgap17.

shADV-252934

Ad-m-ARHGAP17-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Arhgap17 (Rho GTPase activating protein 17). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

5730403H17Rik; Nadrin; Nadrin2; Rich1; Wbp15

Description (Vector)

Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activitor in vitro for RAC1.

Gene ID

70497

Gene Name (Vector)

Rho GTPase activating protein 17

Gene Symbol

Arhgap17

ORF Size (aa)

2175

ORF Size (bp)

2175 bp

RefSeq ID

NM_144529

RefSeq Synonyms

NM_144529, NM_001316713, NM_001122643, NM_001122642, NM_001122641, NM_001122640, BC003259,

Species

mouse

UniGene ID

Mm.403318

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.