mouse ABHD4 shRNA silencing Adenovirus

Name 
Ad-m-ABHD4-shRNA
Cat No 
shADV-251822
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Abhd4.

shADV-251822
Ad-m-ABHD4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Abhd4 (abhydrolase domain containing 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1110035H23Rik; Abh4; AI429574
Description (Vector)
Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity).
Gene ID
105501
Gene Name (Vector)
abhydrolase domain containing 4
Gene Symbol
Abhd4
ORF Size (aa)
1029
ORF Size (bp)
1029 bp
RefSeq ID
NM_134076
RefSeq Synonyms
NM_134076, NM_001205181, BC017532,
Species
mouse
UniGene ID
Mm.28771

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.