mouse GSK3A shRNA silencing Adenovirus

Name 
Ad-m-GSK3A-shRNA
Cat No 
shADV-250850
Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse Gsk3a.

shADV-250850
Ad-m-GSK3A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Gsk3a (glycogen synthase kinase 3 alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2700086H06Rik
Description (Vector)
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transctiption factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth.
Gene ID
606496
Gene Name (Vector)
glycogen synthase kinase 3 alpha
Gene Symbol
Gsk3a
ORF Size (aa)
1473
ORF Size (bp)
1473 bp
RefSeq ID
NM_001031667
RefSeq Synonyms
NM_001031667, BC156581, BC152757,
Species
mouse
UniGene ID
Mm.476745

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.