mouse RBL1 shRNA silencing Adenovirus

Name 
Ad-m-RBL1-shRNA
Cat No 
shADV-250819
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Rbl1.

shADV-250819
Ad-m-RBL1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Rbl1 (retinoblastoma-like 1 (p107)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AW547426; p107; PRB1
Description (Vector)
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
Gene ID
19650
Gene Name (Vector)
retinoblastoma-like 1 (p107)
Gene Symbol
Rbl1
ORF Size (aa)
3192
ORF Size (bp)
3192 bp
RefSeq ID
NM_011249
RefSeq Synonyms
NM_011249, NM_001139516, BC060124,
Species
mouse
UniGene ID
Mm.244671

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.