human MAN2B1 shRNA silencing Adenovirus

Name
Ad-h-MAN2B1-shRNA

Cat No
shADV-230209

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human MAN2B1.

shADV-230209

Ad-h-MAN2B1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MAN2B1 (mannosidase alpha class 2B member 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

LAMAN; MANB

Description (Vector)

MAN2B1 encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

4125

Gene Name (Vector)

mannosidase alpha class 2B member 1

Gene Symbol

MAN2B1

HGNC ID

HGNC:6826

ORF Size (aa)

3036

ORF Size (bp)

3036 bp

RefSeq ID

NM_000528

RefSeq Synonyms

NM_001173498, NM_000528, BC000736,

Species

human

UniGene ID

Hs.356769

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.