human SHMT2 shRNA silencing Adenovirus

Name 
Ad-h-SHMT2-shRNA
Cat No 
shADV-230121
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SHMT2.

shADV-230121
Ad-h-SHMT2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SHMT2 (serine hydroxymethyltransferase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
epididymis secretory sperm binding protein Li 51e; GLY A+; GLYA; glycine auxotroph A, human complement for hamster; glycine hydroxymethyltransferase; HEL-S-51e; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); serine hydroxymethyltransferase, mitochondrial; serine methylase; SHMT; threonine aldolase
Description (eCommerce)
Serine hydroxymethyltransferase (SHMT2) is the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. SHMT2 is primarily responsible for glycine synthesis. SHMT2 activity has been suggested to be the primary source of intracellular glycine [taken from NCBI Entrez Gene (Gene ID: 6472)].
Description (Vector)
SHMT2 encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
Gene ID
6472
Gene Name (eCommerce)
serine hydroxymethyltransferase 2
Gene Name (Vector)
serine hydroxymethyltransferase 2
Gene Symbol
SHMT2
HGNC ID
HGNC:10852
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1515
ORF Size (bp)
1515 bp
Protein Name (eCommerce)
Serine hydroxymethyltransferase, mitochondrial
RefSeq ID
NM_005412
RefSeq Synonyms
NM_005412, NM_001166359, NM_001166358, NM_001166357, NM_001166356, BC044211, BC032584, BC013677, BC011911,
Research Areas
Cytoskeleton,Methyltransferase,Mitochondrion
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
Serine hydroxymethyltransferase (SHMT2) is the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. SHMT2 is primarily responsible for glycine synthesis. SHMT2 activity has been suggested to be the primary source of intracellular glycine [taken from NCBI Entrez Gene (Gene ID: 6472)].
UniGene ID
Hs.719098
UniProt ID (eCommerce)
P34897

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.