human VPS4B shRNA silencing Adenovirus

Name 
Ad-h-VPS4B-shRNA
Cat No 
shADV-227545
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human VPS4B.

shADV-227545
Ad-h-VPS4B-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human VPS4B (vacuolar protein sorting 4 homolog B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
cell migration-inducing 1; cell migration-inducing gene 1 protein; MIG1; Protein SKD1; SKD1; SKD1B; suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; vacuolar protein sorting 4B; vacuolar protein sorting-associated protein 4B; VPS4-2
Description (eCommerce)
VPS4B (vacuolar protein sorting 4 homolog B) belongs to the AAA (adenosine triphosphatases associated with diverse cellular activities) family. It is involved in the multivesicular body (MVB) pathway which is important for cell surface receptor down-regulation and viral budding from host cells. VSP4B plays a role in the disassembly of ESCRTs (endosomal sorting complexes required for transport) on the endosomal membrane during sorting of cargo molecules in the MVB pathway. The mouse paralog of VPS4B is Skd1 (suppressor of K+ transport defect1) and the yeast paralog is Vps4.
Description (Vector)
VPS4B is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
Gene ID
9525
Gene Name (eCommerce)
vacuolar protein sorting 4 homolog B
Gene Name (Vector)
vacuolar protein sorting 4 homolog B
Gene Symbol
VPS4B
HGNC ID
HGNC:10895
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1335
ORF Size (bp)
1335 bp
Protein Name (eCommerce)
Vacuolar protein sorting-associated protein 4B
RefSeq ID
NM_004869
RefSeq Synonyms
NM_004869, BC039574,
Research Areas
Cell Cycle,Cell Division,Host-Virus Interactions,Lysosomal Transport,Lysosome,Signal Transduction,Transport
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,infectious_disease,signal_transduction
Species
human
Target Sentence
VPS4B (vacuolar protein sorting 4 homolog B) belongs to the AAA (adenosine triphosphatases associated with diverse cellular activities) family. It is involved in the multivesicular body (MVB) pathway which is important for cell surface receptor down-regulation and viral budding from host cells. VSP4B plays a role in the disassembly of ESCRTs (endosomal sorting complexes required for transport) on the endosomal membrane during sorting of cargo molecules in the MVB pathway. The mouse paralog of VPS4B is Skd1 (suppressor of K+ transport defect1) and the yeast paralog is Vps4.
UniGene ID
Hs.126550
UniProt ID (eCommerce)
O75351

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.