human VPS25 shRNA silencing Adenovirus

Name 
Ad-h-VPS25-shRNA
Cat No 
shADV-227516
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human VPS25.

shADV-227516
Ad-h-VPS25-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human VPS25 (vacuolar protein sorting 25 homolog). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DERP9; EAP20; FAP20
Description (Vector)
VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 (PubMed 15755741); Kamura et al., 2001 (PubMed 11278625)).(supplied by OMIM, Mar 2008)
Gene ID
84313
Gene Name (Vector)
vacuolar protein sorting 25 homolog
Gene Symbol
VPS25
HGNC ID
HGNC:28122
ORF Size (aa)
531
RefSeq ID
NM_032353
RefSeq Synonyms
NM_032353, BC006282,
Species
human
UniGene ID
Hs.500165

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.