human VIM shRNA silencing Adenovirus

Name 
Ad-h-VIM-shRNA
Cat No 
shADV-227480
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human VIM.

shADV-227480
Ad-h-VIM-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human VIM (vimentin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
epididymis secretory sperm binding protein; vimentin
Description (eCommerce)
Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. [taken from NCBI Entrez Gene (Gene ID: 7431)].
Description (Vector)
VIM encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Gene ID
7431
Gene Name (eCommerce)
vimentin
Gene Name (Vector)
vimentin
Gene Symbol
VIM
HGNC ID
HGNC:12692
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1401
ORF Size (bp)
1401 bp
Protein Name (eCommerce)
Vimentin
RefSeq ID
NM_003380
RefSeq Synonyms
NM_003380, BC066956, BC030573, BC000163,
Research Areas
Apoptosis,Cancer,Cytoskeleton,EGFR Signaling,Host-Virus Interactions,Immunology,MicroRNAs in Cancer,Neurobiology,Neurodevelopment,Signal Transduction,T-cell Receptor Signaling
Research Areas (Faceted)
cancer,genetics,signal_transduction,cell_biology,infectious_disease,immunology,neurobiology,developmental_biology,signal_transduction,immunology
Species
human
Target Sentence
Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. [taken from NCBI Entrez Gene (Gene ID: 7431)].
UniGene ID
Hs.628678
UniProt ID (eCommerce)
P08670

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.