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human USP14 shRNA silencing Adenovirus

Name 
Ad-h-USP14-shRNA
Cat No 
shADV-227286
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human USP14.

shADV-227286
Ad-h-USP14-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human USP14 (ubiquitin specific peptidase 14). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
deubiquitinating enzyme 14; TGT; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14 (tRNA-guanine transglycosylase); ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14
Description (eCommerce)
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 14 (USP14) is a member of this family.
Description (Vector)
USP14 encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene ID
9097
Gene Name (eCommerce)
ubiquitin specific peptidase 14
Gene Name (Vector)
ubiquitin specific peptidase 14
Gene Symbol
USP14
HGNC ID
HGNC:12612
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1485
ORF Size (bp)
1485 bp
Protein Name (eCommerce)
Ubiquitin carboxyl-terminal hydrolase 14
RefSeq ID
NM_005151
RefSeq Synonyms
NM_005151, NM_001037334, BC003556,
Research Areas
Complexome,Neurobiology,Proteasome,Ubiquitination
Research Areas (Faceted)
cell_biology,neurobiology
Species
human
Target Sentence
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 14 (USP14) is a member of this family.
UniGene ID
Hs.707058
UniProt ID (eCommerce)
P54578

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.