human UNC13B shRNA silencing Adenovirus

Name 
Ad-h-UNC13B-shRNA
Cat No 
shADV-227183
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human UNC13B.

shADV-227183
Ad-h-UNC13B-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UNC13B (unc-13 homolog B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
MUNC13; UNC13; Unc13h2
Description (Vector)
UNC13B is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis.
Gene ID
10497
Gene Name (Vector)
unc-13 homolog B
Gene Symbol
UNC13B
HGNC ID
HGNC:12566
ORF Size (aa)
3594
ORF Size (bp)
3594 bp
RefSeq ID
NM_006377
RefSeq Synonyms
NM_006377, NM_001330653, BC111800, BC111781,
Species
human
UniGene ID
Hs.493791

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.