human TYMS shRNA silencing Adenovirus

Name 
Ad-h-TYMS-shRNA
Cat No 
shADV-226935
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human TYMS.

shADV-226935
Ad-h-TYMS-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human TYMS (thymidylate synthetase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HST422; thymidylate synthase; TMS; TS; TSase
Description (eCommerce)
Thymidylate synthetase (TS) catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. TS is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs [taken from NCBI Entrez Gene (Gene ID: 7298)].
Description (Vector)
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
Gene ID
7298
Gene Name (eCommerce)
thymidylate synthetase
Gene Name (Vector)
thymidylate synthetase
Gene Symbol
TYMS
HGNC ID
HGNC:12441
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
942
Protein Name (eCommerce)
Thymidylate synthase
RefSeq ID
NM_001071
RefSeq Synonyms
NM_001071, BC083512, BC013919, BC002567,
Research Areas
Aging,Cancer,Cell Cycle,DNA Damage/Repair,DNA Replication,Host-Virus Interactions,Immunology,Metabolism/Metabolic Process,Methyltransferase,Mitochondrion
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,immunology,metabolism,cell_biology
Species
human
Target Sentence
Thymidylate synthetase (TS) catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. TS is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs [taken from NCBI Entrez Gene (Gene ID: 7298)].
UniGene ID
Hs.592338
UniProt ID (eCommerce)
P04818

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.