human TPP1 shRNA silencing Adenovirus

Name 
Ad-h-TPP1-shRNA
Cat No 
shADV-226283
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human TPP1.

shADV-226283
Ad-h-TPP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human TPP1 (tripeptidyl peptidase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CLN2; GIG1; LPIC; SCAR7; TPP-1
Description (Vector)
TPP1 encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.
Gene ID
1200
Gene Name (Vector)
tripeptidyl peptidase 1
Gene Symbol
TPP1
HGNC ID
HGNC:2073
ORF Size (aa)
1692
ORF Size (bp)
1692 bp
RefSeq ID
NM_000391
RefSeq Synonyms
NM_000391, BC014863,
Species
human
UniGene ID
Hs.523454

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.