human SUCLA2 shRNA silencing Adenovirus

Name 
Ad-h-SUCLA2-shRNA
Cat No 
shADV-224692
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SUCLA2.

shADV-224692
Ad-h-SUCLA2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SUCLA2 (succinate-CoA ligase ADP-forming beta subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A-BETA; A-SCS; ATP-specific succinyl-CoA synthetase subunit beta; ATP-specific succinyl-CoA synthetase, beta subunit; mitochondrial succinyl-CoA ligase ADP-forming subunit beta; MTDPS5; renal carcinoma antigen NY-REN-39; SCS-betaA; succinate--CoA ligase ADP-forming subunit beta, mitochondrial; succinate-CoA ligase beta subunit; succinyl-CoA ligase ADP-forming subunit beta, mitochondrial; succinyl-CoA synthetase beta-A chain
Description (eCommerce)
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. SUCLA2 is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA [taken from NCBI Entrez Gene (Gene ID: 8803)].
Description (Vector)
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
Gene ID
8803
Gene Name (eCommerce)
succinate-CoA ligase ADP-forming beta subunit
Gene Name (Vector)
succinate-CoA ligase ADP-forming beta subunit
Gene Symbol
SUCLA2
HGNC ID
HGNC:11448
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1392
ORF Size (bp)
1392 bp
Protein Name (eCommerce)
Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
RefSeq ID
NM_003850
RefSeq Synonyms
NM_003850, BC027587,
Research Areas
Metabolism/Metabolic Process,Mitochondrion
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. SUCLA2 is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA [taken from NCBI Entrez Gene (Gene ID: 8803)].
UniGene ID
Hs.546323
UniProt ID (eCommerce)
Q9P2R7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.