human STAT2 shRNA silencing Adenovirus

Name
Ad-h-STAT2-shRNA

Cat No
shADV-224537

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human STAT2.

shADV-224537

Ad-h-STAT2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human STAT2 (signal transducer and activator of transcription 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

IMD44; interferon alpha induced transcriptional activator; ISGF-3; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; STAT113

Description (eCommerce)

Signal transducer and activator of transcription 2 (STAT2) is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P52630].

Description (Vector)

STAT2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID

6773

Gene Name (eCommerce)

signal transducer and activator of transcription 2

Gene Name (Vector)

signal transducer and activator of transcription 2

Gene Symbol

STAT2

HGNC ID

HGNC:11363

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2556

ORF Size (bp)

2556 bp

Protein Name (eCommerce)

Signal transducer and activator of transcription 2

RefSeq ID

NM_005419

RefSeq Synonyms

NM_198332, NM_005419, BC051284,

Research Areas

Cancer,Cytokine Signaling,EGFR Signaling,Host-Virus Interactions,IFN Signaling,Immunology,Interleukin Signaling,JAK-STAT Signaling,Response to Virus,Signal Transduction,Transcription Factor/Regulator

Research Areas (Faceted)

cancer,cell_biology,immunology,infectious_disease,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.530595

UniProt ID (eCommerce)

P52630

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.