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human SPRY2 shRNA silencing Adenovirus

Name 
Ad-h-SPRY2-shRNA
Cat No 
shADV-224271
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SPRY2.

shADV-224271
Ad-h-SPRY2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SPRY2 (sprouty RTK signaling antagonist 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
hSPRY2; IGAN3; protein sprouty homolog 2; Spry-2
Description (eCommerce)
Sprouty 2 contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells sprouty 2 is transiently upregulated in response to fibroblast growth factor two. Sprouty 2 is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. Sprouty 2 interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. Sprouty 2 may play a role in alveoli branching during lung development as shown by a similar mouse protein [taken from NCBI Entrez Gene (Gene ID: 10253)].
Description (Vector)
SPRY2 encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.
Gene ID
10253
Gene Name (eCommerce)
sprouty RTK signaling antagonist 2
Gene Name (Vector)
sprouty RTK signaling antagonist 2
Gene Symbol
SPRY2
HGNC ID
HGNC:11270
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
948
Protein Name (eCommerce)
Protein sprouty homolog 2
RefSeq ID
NM_005842
RefSeq Synonyms
NM_005842, NM_001318538, NM_001318537, NM_001318536, BC015745, BC004205,
Research Areas
Apoptosis,Cancer,Cytoskeleton,EGFR Signaling,EGFR Signaling in Cancer,FGF Signaling,Gene Expression,Glioma,JAK-STAT Signaling,MicroRNAs in Cancer,Neurobiology,NGF Signaling,Prostate Cancer,Signal Transduction
Research Areas (Faceted)
cancer,cell_biology,genetics,neurobiology,signal_transduction
Species
human
Target Sentence
Sprouty 2 contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells sprouty 2 is transiently upregulated in response to fibroblast growth factor two. Sprouty 2 is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. Sprouty 2 interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. Sprouty 2 may play a role in alveoli branching during lung development as shown by a similar mouse protein [taken from NCBI Entrez Gene (Gene ID: 10253)].
UniGene ID
Hs.18676
UniProt ID (eCommerce)
O43597

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.