human SMC1A shRNA silencing Adenovirus

Name 
Ad-h-SMC1A-shRNA
Cat No 
shADV-223732
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SMC1A.

shADV-223732
Ad-h-SMC1A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SMC1A (structural maintenance of chromosomes 1A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CDLS2; DXS423E; epididymis secretory sperm binding protein; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC-1A; SMC1alpha; SMC-1-alpha; SMC1L1; SMCB; structural maintenance of chromosomes protein 1A
Description (eCommerce)
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.
Description (Vector)
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
Gene ID
8243
Gene Name (eCommerce)
structural maintenance of chromosomes 1A
Gene Name (Vector)
structural maintenance of chromosomes 1A
Gene Symbol
SMC1A
HGNC ID
HGNC:11111
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3702
ORF Size (bp)
3702 bp
Protein Name (eCommerce)
Structural maintenance of chromosomes protein 1A
RefSeq ID
NM_006306
RefSeq Synonyms
NM_006306, NM_001281463, BC112127,
Research Areas
Cell Cycle,Cell Division,Chromatin Remodeling,DNA Damage/Repair,Gene Expression,Homologous Recombination,Immunology,Kinase/Phosphatase,Meiosis,mRNA Processing,mRNA Splicing,Neurobiology,Phospho-specific,RNA Processing,Signal Transduction,Spliceosome
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,immunology,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.
UniGene ID
Hs.211602
UniProt ID (eCommerce)
Q14683

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.