human SLC29A1 shRNA silencing Adenovirus

Name 
Ad-h-SLC29A1-shRNA
Cat No 
shADV-223326
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SLC29A1.

shADV-223326
Ad-h-SLC29A1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SLC29A1 (solute carrier family 29 member 1 (Augustine blood group)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ENT1; Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; equilibrative nucleoside transporter 1; nucleoside transporter, es-type; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1
Description (eCommerce)
Equilibrative nucleoside transporter 1 (ENT1) mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine and is inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99808].
Description (Vector)
SLC29A1 is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
Gene ID
2030
Gene Name (eCommerce)
solute carrier family 29 member 1 (Augustine blood group)
Gene Name (Vector)
solute carrier family 29 member 1 (Augustine blood group)
Gene Symbol
SLC29A1
HGNC ID
HGNC:11003
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1371
ORF Size (bp)
1371 bp
Protein Name (eCommerce)
Equilibrative nucleoside transporter 1
RefSeq ID
NM_004955
RefSeq Synonyms
NM_004955, NM_001304466, NM_001304465, NM_001304463, NM_001304462, NM_001078177, NM_001078176, NM_001078175, NM_001078174, BC008954, BC001382,
Research Areas
Hypoxia,Neurobiology,Transport
Research Areas (Faceted)
cell_biology,neurobiology
Species
human
Target Sentence
Equilibrative nucleoside transporter 1 (ENT1) mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine and is inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99808].
UniGene ID
Hs.25450
UniProt ID (eCommerce)
Q99808

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.