human RPE65 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human RPE65.
shADV-221550
Ad-h-RPE65-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human RPE65 (RPE65, retinoid isomerohydrolase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
BCO3; LCA2; mRPE65; rd12; RP20; sRPE65
Description (Vector)
RPE65 encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
Gene ID
6121
Gene Name (Vector)
RPE65, retinoid isomerohydrolase
Gene Symbol
RPE65
HGNC ID
HGNC:10294
ORF Size (aa)
1602
ORF Size (bp)
1602 bp
RefSeq ID
NM_000329
RefSeq Synonyms
NM_000329, BC075036, BC075035,
Species
human
UniGene ID
Hs.2133
Recently Viewed Products
AAV-h-RUFY1-shRNA
Cat No: shAAV-222105
AAV-h-RDBP-shRNA
Cat No: shAAV-220956
Ad-h-PGDS-shRNA
Cat No: shADV-218730
AAV-h-RPE65-shRNA
Cat No: shAAV-221550
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.