mouse RPE65 Over-expression AAV

Name 
AAV-m-RPE65
Cat No 
AAV-270828
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Rpe65.

AAV-270828
AAV-m-RPE65

Ready-to-use AAV expressing Mouse Rpe65 (retinal pigment epithelium 65). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
65kDa; A930029L06Rik; LCA2; Mord1; rd12; RP20
Description (Vector)
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
Gene ID
19892
Gene Name (Vector)
retinal pigment epithelium 65
Gene Symbol
Rpe65
ORF Size (aa)
1602
ORF Size (bp)
1602 bp
RefSeq ID
NM_029987
RefSeq Synonyms
NM_029987, BC130028,
Species
mouse
UniGene ID
Mm.131708

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.