human RPA2 shRNA silencing Adenovirus

Name 
Ad-h-RPA2-shRNA
Cat No 
shADV-221532
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RPA2.

shADV-221532
Ad-h-RPA2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RPA2 (replication protein A2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32
Description (eCommerce)
Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
Gene ID
6118
Gene Name (eCommerce)
replication protein A2
Gene Name (Vector)
replication protein A2
Gene Symbol
RPA2
HGNC ID
HGNC:10290
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
813
ORF Size (bp)
813 bp
Protein Name (eCommerce)
Replication protein A 32 kDa subunit
RefSeq ID
NM_002946
RefSeq Synonyms
NM_002946, NM_001297558, NM_001286076, BC021257, BC012157, BC001630,
Research Areas
Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Homologous Recombination,Neurobiology,Phospho-specific,Replication,Signal Transduction,Telomeric Maintenance
Research Areas (Faceted)
signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,neurobiology,cell_biology,cell_cycle,genetics
Species
human
Target Sentence
Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
UniGene ID
Hs.79411
UniProt ID (eCommerce)
P15927

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.