human RFC3 shRNA silencing Adenovirus

Name 
Ad-h-RFC3-shRNA
Cat No 
shADV-221038
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RFC3.

shADV-221038
Ad-h-RFC3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RFC3 (replication factor C subunit 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A1 38 kDa subunit; activator 1 38 kDa subunit; activator 1 subunit 3; replication factor C (activator 1) 3, 38kDa; replication factor C 38 kDa subunit; replication factor C subunit 3; RF-C 38 kDa subunit; RFC, 38 kD subunit; RFC38
Description (eCommerce)
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC3 is the 38 kDa subunit of RFC that is essential for the interaction between RFC1 and the core formed by RFC2, RFC4, and RFC5.
Description (Vector)
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Gene ID
5983
Gene Name (eCommerce)
replication factor C subunit 3
Gene Name (Vector)
replication factor C subunit 3
Gene Symbol
RFC3
HGNC ID
HGNC:9971
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1071
Protein Name (eCommerce)
Replication factor C subunit 3
RefSeq ID
NM_181558
RefSeq Synonyms
NM_181558, NM_002915, BC000149,
Research Areas
Cancer,Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Homologous Recombination,Replication,Signal Transduction,Telomeric Maintenance
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,genetics
Species
human
Target Sentence
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC3 is the 38 kDa subunit of RFC that is essential for the interaction between RFC1 and the core formed by RFC2, RFC4, and RFC5.
UniGene ID
Hs.115474
UniProt ID (eCommerce)
P40938

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.