human RANGAP1 shRNA silencing Adenovirus

Name 
Ad-h-RANGAP1-shRNA
Cat No 
shADV-220709
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RANGAP1.

shADV-220709
Ad-h-RANGAP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RANGAP1 (Ran GTPase activating protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Fug1; ran GTPase-activating protein 1; RANGAP; RanGAP1; SD; segregation distorter homolog; segregation distortion
Description (eCommerce)
The regulation of GTPase activity is achieved via activators called GEFs (guanine-nucleotide exchange factors) and inactivators called GAPs (GTPase-activating proteins). Ran-specific GTPase-activating protein 1 (RanGAP1) induces the GTPase activity of the RAN small GTPase. The Ran GTPase plays a role in spatial information and nucleocytoplasmic transport. RanGAP1 is the antagonist of the GEF RCC1 which keeps RAN in the active GTP-bound state.
Description (Vector)
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
Gene ID
5905
Gene Name (eCommerce)
Ran GTPase activating protein 1
Gene Name (Vector)
Ran GTPase activating protein 1
Gene Symbol
RANGAP1
HGNC ID
HGNC:9854
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1764
ORF Size (bp)
1764 bp
Protein Name (eCommerce)
Ran GTPase-activating protein 1
RefSeq ID
NM_002883
RefSeq Synonyms
NM_002883, NM_001317930, NM_001278651, BC041396, BC014044,
Research Areas
Cell Cycle,Cytoskeleton,Host-Virus Interactions,RNA Transport,Signal Transduction,Transport
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,infectious_disease,signal_transduction,transcription_translation
Species
human
Target Sentence
The regulation of GTPase activity is achieved via activators called GEFs (guanine-nucleotide exchange factors) and inactivators called GAPs (GTPase-activating proteins). Ran-specific GTPase-activating protein 1 (RanGAP1) induces the GTPase activity of the RAN small GTPase. The Ran GTPase plays a role in spatial information and nucleocytoplasmic transport. RanGAP1 is the antagonist of the GEF RCC1 which keeps RAN in the active GTP-bound state.
UniGene ID
Hs.183800
UniProt ID (eCommerce)
P46060

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.