human PYGL shRNA silencing Adenovirus

Name 
Ad-h-PYGL-shRNA
Cat No 
shADV-220448
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PYGL.

shADV-220448
Ad-h-PYGL-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PYGL (glycogen phosphorylase L). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
glycogen phosphorylase, liver form; GSD6; phosphorylase, glycogen, liver
Description (eCommerce)
Glycogen phosphorylase, liver form, (PYGL) encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues [taken from NCBI Entrez Gene (Gene ID: 5836)].
Description (Vector)
PYGL encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID
5836
Gene Name (eCommerce)
glycogen phosphorylase L
Gene Name (Vector)
glycogen phosphorylase L
Gene Symbol
PYGL
HGNC ID
HGNC:9725
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2541
ORF Size (bp)
2541 bp
Protein Name (eCommerce)
Glycogen phosphorylase, liver form
RefSeq ID
NM_002863
RefSeq Synonyms
NM_002863, NM_001163940, BC110791, BC095850, BC082229, BC009895,
Research Areas
Insulin Signaling,Metabolism/Metabolic Process,Signal Transduction,TNF Signaling
Research Areas (Faceted)
cell_biology,metabolism,signal_transduction
Species
human
Target Sentence
Glycogen phosphorylase, liver form, (PYGL) encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues [taken from NCBI Entrez Gene (Gene ID: 5836)].
UniGene ID
Hs.282417
UniProt ID (eCommerce)
P06737

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.