human PUS10 shRNA silencing Adenovirus

Name
Ad-h-PUS10-shRNA

Cat No
shADV-220403

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human PUS10.

shADV-220403

Ad-h-PUS10-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PUS10 (pseudouridylate synthase 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

CCDC139; DOBI

Description (Vector)

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 (PubMed 17900615)).(supplied by OMIM, May 2009)

Gene ID

150962

Gene Name (Vector)

pseudouridylate synthase 10

Gene Symbol

PUS10

HGNC ID

HGNC:26505

ORF Size (aa)

1590

ORF Size (bp)

1590 bp

RefSeq ID

NM_144709

RefSeq Synonyms

NM_144709, NM_001322127, NM_001322124, NM_001322123, BC101706, BC101680,

Species

human

UniGene ID

Hs.368348

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.