human PTPN12 shRNA silencing Adenovirus

Name 
Ad-h-PTPN12-shRNA
Cat No 
shADV-220309
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PTPN12.

shADV-220309
Ad-h-PTPN12-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PTPN12 (protein tyrosine phosphatase, non-receptor type 12). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
protein-tyrosine phosphatase G1; PTPG1; PTP-PEST; tyrosine-protein phosphatase non-receptor type 12
Description (eCommerce)
PTPN12 is a cytosolic protein tyrosine phosphatase (PTP) that contains a C-terminal PEST motif and plays an important role in cytoskeletal organization, immune cell activation, apoptosis, and embryonic development. PTPN12 appears to contribute to these cellular processes through its interaction with a variety of signaling and cytoskeletal-associated proteins. The recent discovery that PTPN12 is modulated by caspase-3 cleavage has led to the analysis of the role of PTPN12 and other PTPs as regulators of apoptosis.
Description (Vector)
PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
5782
Gene Name (eCommerce)
protein tyrosine phosphatase, non-receptor type 12
Gene Name (Vector)
protein tyrosine phosphatase, non-receptor type 12
Gene Symbol
PTPN12
HGNC ID
HGNC:9645
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2343
ORF Size (bp)
2343 bp
Protein Name (eCommerce)
Tyrosine-protein phosphatase non-receptor type 12
RefSeq ID
NM_002835
RefSeq Synonyms
NM_002835, NM_001131009, NM_001131008, BC050008,
Research Areas
EGFR Signaling,Immunology,Kinase/Phosphatase,Phosphatase,Signal Transduction,T-cell Receptor Signaling
Research Areas (Faceted)
signal_transduction,immunology,cell_biology,signal_transduction,immunology
Species
human
Target Sentence
PTPN12 is a cytosolic protein tyrosine phosphatase (PTP) that contains a C-terminal PEST motif and plays an important role in cytoskeletal organization, immune cell activation, apoptosis, and embryonic development. PTPN12 appears to contribute to these cellular processes through its interaction with a variety of signaling and cytoskeletal-associated proteins. The recent discovery that PTPN12 is modulated by caspase-3 cleavage has led to the analysis of the role of PTPN12 and other PTPs as regulators of apoptosis.
UniGene ID
Hs.61812
UniProt ID (eCommerce)
Q05209

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.