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human PTCH1 shRNA silencing Adenovirus

Name 
Ad-h-PTCH1-shRNA
Cat No 
shADV-220214
Availability
Immediate
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PTCH1.

shADV-220214
Ad-h-PTCH1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PTCH1 (patched 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
BCNS; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11
Description (Vector)
PTCH1 encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. PTCH1 functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.
Gene ID
5727
Gene Name (Vector)
patched 1
Gene Symbol
PTCH1
HGNC ID
HGNC:9585
ORF Size (aa)
4341
ORF Size (bp)
4341 bp
RefSeq ID
NM_000264
RefSeq Synonyms
NM_001083607, NM_001083606, NM_001083605, NM_001083604, NM_001083603, NM_001083602, NM_000264, BC152919, BC043542,
Species
human
UniGene ID
Hs.638946

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.