human PSME1 shRNA silencing Adenovirus

Name
Ad-h-PSME1-shRNA

Cat No
shADV-220175

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human PSME1.

shADV-220175

Ad-h-PSME1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PSME1 (proteasome activator subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

11S regulator complex subunit alpha; 29-kD MCP activator subunit; activator of multicatalytic protease subunit 1; epididymis secretory sperm binding protein Li 129m; HEL-S-129m; IFI5111; IGUP I-5111; interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; PA28A; PA28alpha; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; proteasome activator complex subunit 1; REGalpha; REG-alpha

Description (eCommerce)

PSME1 [proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)] is the alpha subunit of the 11S regulator. The 11S regulator is part of the immunoproteasome, a modified proteasome involved in the processing of class I MHC peptides [taken from NCBI Entrez Gene (Gene ID: 5720)].

Description (Vector)

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

Gene ID

5720

Gene Name (eCommerce)

proteasome activator subunit 1

Gene Name (Vector)

proteasome activator subunit 1

Gene Symbol

PSME1

HGNC ID

HGNC:9568

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

750

Protein Name (eCommerce)

Proteasome activator complex subunit 1

RefSeq ID

NM_006263

RefSeq Synonyms

NM_176783, NM_006263, NM_001281529, NM_001281528, BC007503, BC000352,

Research Areas

Antigen Processing and Presentation,Apoptosis,Cell Cycle,Cell Division,Complexome,DNA Damage/Repair,Gene Expression,Host-Virus Interactions,Immunology,Metabolism/Metabolic Process,Proteasome,RNA Processing,Ubiquitination

Research Areas (Faceted)

immunology,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,metabolism

Species

human

Target Sentence

UniGene ID

Hs.75348

UniProt ID (eCommerce)

Q06323

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.