human PSMB10 shRNA silencing Adenovirus

Name 
Ad-h-PSMB10-shRNA
Cat No 
shADV-220118
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PSMB10.

shADV-220118
Ad-h-PSMB10-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PSMB10 (proteasome subunit beta 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
beta2i; LMP10; MECL1
Description (Vector)
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
Gene ID
5699
Gene Name (Vector)
proteasome subunit beta 10
Gene Symbol
PSMB10
HGNC ID
HGNC:9538
ORF Size (aa)
822
RefSeq ID
NM_002801
RefSeq Synonyms
NM_002801, BC052369, BC017198,
Species
human
UniGene ID
Hs.9661

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.