human PRCP shRNA silencing Adenovirus

Name 
Ad-h-PRCP-shRNA
Cat No 
shADV-219745
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PRCP.

shADV-219745
Ad-h-PRCP-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PRCP (prolylcarboxypeptidase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HUMPCP; PCP
Description (Vector)
PRCP is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene ID
5547
Gene Name (Vector)
prolylcarboxypeptidase
Gene Symbol
PRCP
HGNC ID
HGNC:9344
ORF Size (aa)
1491
ORF Size (bp)
1491 bp
RefSeq ID
NM_005040
RefSeq Synonyms
NM_199418, NM_005040, NM_001319214, BC001500,
Species
human
UniGene ID
Hs.523936

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.