human PPM1G shRNA silencing Adenovirus

Name 
Ad-h-PPM1G-shRNA
Cat No 
shADV-219582
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PPM1G.

shADV-219582
Ad-h-PPM1G-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PPM1G (protein phosphatase, Mg2+/Mn2+ dependent 1G). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
74
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
PP2CG; PP2CGAMMA; PP2C-gamma; PPP2CG; protein phosphatase 1C; protein phosphatase 1G; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma
Description (eCommerce)
Members of the protein phosphatase 2C (PP2C) family of isoenzymes are serine/threonine protein phosphatases that act as monomers, are magnesium or manganese dependent, and are structurally distinct from PP1, PP2A, and PP2B protein phosphatases. There are 14 mammalian PP2C genes that encode for members of the PP2C family of isoenzymes. Members of this family include PP2C alpha (PPM1A), PP2C beta (PPM1B), PP2C gamma (PPM1G), and PP2C delta (PPM1D). Because PPM1G has been shown to bind and dephosphorylate histones H2A and H2B, it appears to play a role in chromatin remodeling in response to DNA damage.
Description (Vector)
PPM1G is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.
Gene ID
5496
Gene Name (eCommerce)
protein phosphatase, Mg2+/Mn2+ dependent 1G
Gene Name (Vector)
protein phosphatase, Mg2+/Mn2+ dependent 1G
Gene Symbol
PPM1G
HGNC ID
HGNC:9278
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1641
ORF Size (bp)
1641 bp
Protein Name (eCommerce)
Protein phosphatase 1G
RefSeq ID
NM_177983
RefSeq Synonyms
NM_177983, BC022061, BC000057,
Research Areas
Cell Cycle,Kinase/Phosphatase,mRNA Processing,Phosphatase,RNA Processing
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
Members of the protein phosphatase 2C (PP2C) family of isoenzymes are serine/threonine protein phosphatases that act as monomers, are magnesium or manganese dependent, and are structurally distinct from PP1, PP2A, and PP2B protein phosphatases. There are 14 mammalian PP2C genes that encode for members of the PP2C family of isoenzymes. Members of this family include PP2C alpha (PPM1A), PP2C beta (PPM1B), PP2C gamma (PPM1G), and PP2C delta (PPM1D). Because PPM1G has been shown to bind and dephosphorylate histones H2A and H2B, it appears to play a role in chromatin remodeling in response to DNA damage.
UniGene ID
Hs.643951
UniProt ID (eCommerce)
O15355

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.