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human POLR2J2 shRNA silencing Adenovirus

Name 
Ad-h-POLR2J2-shRNA
Cat No 
shADV-219406
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human POLR2J2.

shADV-219406
Ad-h-POLR2J2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human POLR2J2 (RNA polymerase II subunit J2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HRPB11B; RPB11b1
Description (Vector)
POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo.
Gene ID
246721
Gene Name (Vector)
RNA polymerase II subunit J2
Gene Symbol
POLR2J2
HGNC ID
HGNC:23208
ORF Size (aa)
351
RefSeq ID
NM_032959
RefSeq Synonyms
NM_032959, BC086857,
Species
human
UniGene ID
Hs.700609

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.