human POLH shRNA silencing Adenovirus

Name 
Ad-h-POLH-shRNA
Cat No 
shADV-219367
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human POLH.

shADV-219367
Ad-h-POLH-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human POLH (DNA polymerase eta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DNA polymerase eta; polymerase (DNA directed), eta; polymerase (DNA) eta; RAD30; RAD30 homolog A; RAD30A; xeroderma pigmentosum variant type protein; XPV; XP-V
Description (eCommerce)
PolH is a DNA polymerase specifically involved in DNA repair and somatic hypermutation. Defects in PolH result in a variant type of xeroderma pigmentosum, a disease characterized by sunlight hypersensitivity, increased incidence of cancer, and neurological abnormalities.
Description (Vector)
POLH encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum.
Gene ID
5429
Gene Name (eCommerce)
DNA polymerase eta
Gene Name (Vector)
DNA polymerase eta
Gene Symbol
POLH
HGNC ID
HGNC:9181
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1245
ORF Size (bp)
1245 bp
Protein Name (eCommerce)
DNA polymerase eta
RefSeq ID
NM_006502
RefSeq Synonyms
NM_006502, NM_001291970, NM_001291969, BC015742,
Research Areas
DNA Damage/Repair,DNA Replication,Fanconi anemia
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
PolH is a DNA polymerase specifically involved in DNA repair and somatic hypermutation. Defects in PolH result in a variant type of xeroderma pigmentosum, a disease characterized by sunlight hypersensitivity, increased incidence of cancer, and neurological abnormalities.
UniGene ID
Hs.655467
UniProt ID (eCommerce)
Q9Y253

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.