human POLA2 shRNA silencing Adenovirus

Name 
Ad-h-POLA2-shRNA
Cat No 
shADV-219343
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human POLA2.

shADV-219343
Ad-h-POLA2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human POLA2 (DNA polymerase alpha 2, accessory subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DNA polymerase alpha 70 kDa subunit; DNA polymerase alpha subunit B; polymerase (DNA directed), alpha 2 (70kD subunit); polymerase (DNA directed), alpha 2, accessory subunit; polymerase (DNA) alpha 2, accessory subunit; polymerase (DNA-directed), alpha (70kD)
Description (eCommerce)
DNA polymerase alpha is a four-subunit enzyme that exhibits DNA polymerase and primase activity. The four subunits of the heterotetrameric DNA pol alpha are A, B, C, and D. Subunit A displays the catalytic activity while the C and D subunits exhibit the primase activity. DNA polymerase (pol) alpha subunit B (PolA2) is the 70 kDa subunit that may serve to couple the DNA pol alpha-primase complex to the initiation/elongation machinery. DNA pol alpha is the only polymerase in eukaryotic cells that is capable of starting chains de novo.
Gene ID
23649
Gene Name (eCommerce)
DNA polymerase alpha 2, accessory subunit
Gene Name (Vector)
DNA polymerase alpha 2, accessory subunit
Gene Symbol
POLA2
HGNC ID
HGNC:30073
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1797
ORF Size (bp)
1797 bp
Protein Name (eCommerce)
DNA polymerase alpha subunit B
RefSeq ID
NM_002689
RefSeq Synonyms
NM_002689, BC002990, BC001347,
Research Areas
Cell Cycle,DNA Damage/Repair,DNA Replication,Immunology,Replication,Telomeric Maintenance
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation,immunology
Species
human
Target Sentence
DNA polymerase alpha is a four-subunit enzyme that exhibits DNA polymerase and primase activity. The four subunits of the heterotetrameric DNA pol alpha are A, B, C, and D. Subunit A displays the catalytic activity while the C and D subunits exhibit the primase activity. DNA polymerase (pol) alpha subunit B (PolA2) is the 70 kDa subunit that may serve to couple the DNA pol alpha-primase complex to the initiation/elongation machinery. DNA pol alpha is the only polymerase in eukaryotic cells that is capable of starting chains de novo.
UniGene ID
Hs.201897
UniProt ID (eCommerce)
Q14181

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.