human PIK3CA shRNA silencing Adenovirus

Name 
Ad-h-PIK3CA-shRNA
Cat No 
shADV-218945
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PIK3CA.

shADV-218945
Ad-h-PIK3CA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CLOVE; CWS5; MCAP; MCM; MCMTC; p110-alpha; PI3K; PI3K-alpha
Description (Vector)
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene ID
5290
Gene Name (Vector)
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Gene Symbol
PIK3CA
HGNC ID
HGNC:8975
ORF Size (aa)
3207
ORF Size (bp)
3207 bp
RefSeq ID
NM_006218
RefSeq Synonyms
NM_006218, BC113603, BC113601,
Species
human
UniGene ID
Hs.553498

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.