human PEG10 shRNA silencing Adenovirus

Name
Ad-h-PEG10-shRNA

Cat No
shADV-218604

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PEG10.

shADV-218604

Ad-h-PEG10-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PEG10 (paternally expressed 10). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

EDR; embryonal carcinoma differentiation regulated; Embryonal carcinoma differentiation-regulated protein; HB-1; mammalian retrotransposon-derived 2; mammalian retrotransposon-derived protein 2; Mar2; Mart2; MEF3 like 1; MEF3L; MEF3-like protein 1; myelin expression factor 3-like protein 1; paternally expressed gene 10 protein; retrotransposon gag domain-containing protein 3; retrotransposon Gag like 2; retrotransposon-derived gag-like polyprotein; retrotransposon-derived protein PEG10; RGAG3; RTL2; SIRH1; Sushi-Ichi retrotransposon homolog 1; ty3/Gypsy-like protein

Description (eCommerce)

Retrotransposon-derived protein PEG10 prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. PEG10 may also have a role in cell growth promotion and hepatoma formation. It inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1 [taken from UniProtKB (Entry: Q86TG7)].

Description (Vector)

This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants, as well as from use of upstream non-AUG (CUG) start codon, have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas.

Gene ID

23089

Gene Name (eCommerce)

paternally expressed 10

Gene Name (Vector)

paternally expressed 10

Gene Symbol

PEG10

HGNC ID

HGNC:14005

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

978

Protein Name (eCommerce)

Retrotransposon-derived protein PEG10

RefSeq ID

NM_001040152

RefSeq Synonyms

NM_015068, NM_001184962, NM_001184961, NM_001172438, NM_001172437, NM_001040152, BC050659,

Research Areas

Apoptosis,Zinc-finger

Research Areas (Faceted)

cell_biology

Species

human

Target Sentence

UniGene ID

Hs.147492

UniProt ID (eCommerce)

Q86TG7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.