human PAFAH1B1 shRNA silencing Adenovirus

Name
Ad-h-PAFAH1B1-shRNA

Cat No
shADV-218127

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PAFAH1B1.

shADV-218127

Ad-h-PAFAH1B1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

LIS1; LIS-1; LIS2; lissencephaly 1 protein; Lissencephaly-1 protein; MDCR; MDS; NudF; PAF acetylhydrolase 45 kDa subunit; PAFAH; PAF-AH 45 kDa subunit; PAFAH alpha; PAF-AH alpha; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa); platelet-activating factor acetylhydrolase IB subunit alpha; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD); platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)

Description (eCommerce)

Lissencephaly-1 protein (LIS-1) was identified as a gene associated with Miller-Dieker lissencephaly syndrome, an autosomal dominant disorder characterized by microcephaly along with anomalies of the kidney, heart, and gastrointestinal tract. LIS-1 is the non-catalytic alpha subunit of the intracellular isoform of platelet-activating factor acetylhydrolase. LIS-1 plays an important role in neuron migration during brain development and fibroblast migration during wound healing. Its role in migration is related to its function in regulating dynein-mediated microtubule sliding.

Description (Vector)

This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.

Gene ID

5048

Gene Name (eCommerce)

platelet activating factor acetylhydrolase 1b regulatory subunit 1

Gene Name (Vector)

platelet activating factor acetylhydrolase 1b regulatory subunit 1

Gene Symbol

PAFAH1B1

HGNC ID

HGNC:8574

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1233

Protein Name (eCommerce)

Platelet-activating factor acetylhydrolase IB subunit alpha

RefSeq ID

NM_000430

RefSeq Synonyms

NM_000430, BC064638,

Research Areas

Cell Cycle,Cell Division,Cytoskeleton,Neurobiology,Neurodevelopment,Neurogenesis,Signal Transduction,Transport,Wnt Signaling

Research Areas (Faceted)

cell_biology,cell_cycle,developmental_biology,genetics,neurobiology,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.77318

UniProt ID (eCommerce)

P43034

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.