Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human P4HTM shRNA silencing Adenovirus

Name 
Ad-h-P4HTM-shRNA
Cat No 
shADV-218084
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human P4HTM.

shADV-218084
Ad-h-P4HTM-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human P4HTM (prolyl 4-hydroxylase, transmembrane). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
EGLN4; HIFPH4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4; P4H with transmembrane domain; P4H-TM; PH4; PH-4; PHD4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; transmembrane prolyl 4-hydroxylase
Description (eCommerce)
PHD4 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity.
Description (Vector)
The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified.
Gene ID
54681
Gene Name (eCommerce)
prolyl 4-hydroxylase, transmembrane
Gene Name (Vector)
prolyl 4-hydroxylase, transmembrane
Gene Symbol
P4HTM
HGNC ID
HGNC:28858
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1272
ORF Size (bp)
1272 bp
Protein Name (eCommerce)
Transmembrane prolyl 4-hydroxylase
RefSeq ID
NM_177939
RefSeq Synonyms
NM_177939, NM_177938, BC047566, BC011710,
Research Areas
Hypoxia,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology,metabolism
Species
human
Target Sentence
PHD4 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity.
UniGene ID
Hs.654944
UniProt ID (eCommerce)
Q9NXG6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.